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I putted all the directions in Lab exercise word doc. The other needed documents are also attached. I need to upload one more ppt. _lab_exercise.docx _lab_exercise.docx _cm_ruler.pdf inhibition_of_bacterial_growth.docx protist_diversity.docx plant_anatomy_and_morphology_lab.pdf Unformatted Attachment Preview Prokaryotes Lab Exercise Introduction Below is a link to a Power Point that will give you an introduction this lab exercise. The introduction will provide you with important context for conducting this lab as well as clearly identify the objectives for this lab. • PowerPoint - Caption for narrations available in the notes section: Link to Prokaryotes and Protists Intro Power Point ( I will attached) For this lab you will need: • A metric ruler you. If you dont have one, you can print the file linked here - dont spend money on this lab! Just make sure to print at 100\% or actual size to ensure the proper measurements. Making no assumptions about your math abilities, you may require a calculator THE HERE LINK IS: 15 cm by 1 mm • 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net Inhibition of Bacterial Growth Excercise This Learning Module item should open in a new window. If not, then click the link below. Click to Launch ( I WILL ATTACH IT: Inhibition of bacterial growth) End of Lab Wrap Up Please ensure that all of your notes and materials are neatly organized to assist you in taking the end of unit assessment. Like any good scientist we expect that you will be able to use your collected data and notes to help you make amazing connections between your material. Those students with neatly organized notes will find success on the end of unit assessment. You will find that assessment located in the root folder for the unit. Please close this module and move on to the next module in this learning unit. If this is the last module, then prepare for your assessment and good luck! Protists Lab Exercise Introduction Below is a link to a Power Point that will give you an introduction this lab exercise. The introduction will provide you with important context for conducting this lab as well as clearly identify the objectives for this lab. • PowerPoint - Caption for narrations available in the notes section: Link to Protists Intro Power Point ( I will attach) Protist Diversity Exercise This Learning Module item should open in a new window. If not, then click the link below. Click to Launch ( I will attach) Plantae Lab Exercise 1 - Anatomy and Physiology Introduction Below is a link to a Power Point that will give you an introduction this lab exercise. The introduction will provide you with important context for conducting this lab as well as clearly identify the objectives for this lab. PowerPoint - Caption for narrations available in the notes section: Link to Plant Anatomy and Morphology Intro Power Point Plants play important roles in our everyday lives. Plants are shelter, medicine, clothing, and as an important food sources. Plants are also essential in the oxygen, water, and, nitrogen cycles that support life on Earth.In the distribution of life on this planet, plants provide important habitats and food sources for every animal that roams the Earth. Even if an animal doesnt depend on a plant directly for food, something in the food chain does. In our discovery of plants, we will discover various plant organs that grow both below and above the ground. Plants also have a fascinating internal anatomy. The first purpose of this lab is to familiarize you with plant cells,organs, and tissues. Secondly we hope this lab exercise will increase your appreciation for botany as food and a part of your life. ( I will attach the PPT) Materials For this exercise you will need to make an excursion to your local grocery store or to your pantry if it’s well stocked with fresh vegetables. Don’t worry you won’t be buying anything just examining them in the store (unless you want to buy them in which case you can enjoy a nice snack made of your specimens at the end of this lab). • • • You will need 8 specimens from grocery store or your pantry. You will also need to go outside and find three stems and two root systems. You will also need six different leaf types (PS - you should probably consult Google to determine what poison oak and poison ivy look like before you do this - or wear safety equipment) Remember, safety in lab is YOUR responsibility! Directions • Attached Files • Plant Anatomy and Morphology Lab.pdf Plant Anatomy and Morphology Lab.pdf - Alternative Formats (1.332 MB) Please see the attached lab document to guide you through this lab experience. ( I will attach the PDF) Materials For this lab you will need: • • Access to plants. A neighborhood walk should do fine; however, a wildlife area, a botanical garden, or even a garden store will have many of the items you are looking for. If you are unable to traverse outside for any reason you may complete this exercise using your computer and downloading images. Please either contact me or with our office of disability services to help process that accommodation. They can be reached at 972-273-3165. A digital camera (does not have to be fancy) or if you do not have one - drawing paper and lots of time. Directions Attached Files • Plant Scavenger Hunt Template.pptx Plant Scavenger Hunt Template.pptx - Alternative Formats (49.839 KB) For this lab you will be going on a plant diversity scavenger hunt/bingo. I will allow you some degree of freedom on how you upload your final product. I recommend power/point and I have provided a template for you. However, if you have another format, I will support you. The one stipulation is that it is uploaded as a file, not as a link or a shared file. If you do an amazing job your scavenger hunt may be subjected to bonus points. Step One: Go on a plant walk (or two) and see how many of these items you can find. If you are unable to find them, you can get an image from the internet but not all of your images can be from downloads. Two things here first over 50\% of your images must be original and if you do have downloads they CANNOT be from my lectures or lab materials. Step Two: for each item note (a) general location e.g. Central Park NY, NE side corner of 85th St. and 5th Ave, (b) note general observations e.g. healthy population, plentiful, raccoons were feasting on abandoned twinkles in the area, (c) indicate if the image is original or if it is downloaded - include source, and (d) general taxonomy Domain, Kingdom, Phylum and common name. Must have all four of those. Any additional info beyond that is cool! Step Three: Supplement any of your missing categories with downloads (see above, must be original and no more than 49\% of your offerings) Step Four: Put your plant scavenger hunt items into the power point template or some other creative uploadable form (PDF, PPT, PPTX or JPG only). You will upload this in the final question of your end of lab quiz. Thats it ... enjoy nature. Scavenger Hunt Checklist Y/N Location Any Bryophyte Any Fern/Fern Allies Fern Sori Any coniferophyta Any non-conifer gymnosperm A female cone Any flowering plant A monocot A eudicot Leaf with parallel venation Leaf with palmate/pinnate venation A fruit - fleshy for animal dispersal A fruit - dry Original Image Taxonomy General notes and observations Prokaryotes Lab Exercise Introduction Below is a link to a Power Point that will give you an introduction this lab exercise. The introduction will provide you with important context for conducting this lab as well as clearly identify the objectives for this lab. • PowerPoint - Caption for narrations available in the notes section: Link to Prokaryotes and Protists Intro Power Point ( I will attached) For this lab you will need: • A metric ruler you. If you dont have one, you can print the file linked here - dont spend money on this lab! Just make sure to print at 100\% or actual size to ensure the proper measurements. Making no assumptions about your math abilities, you may require a calculator THE HERE LINK IS: 15 cm by 1 mm • 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net Inhibition of Bacterial Growth Excercise This Learning Module item should open in a new window. If not, then click the link below. Click to Launch ( I WILL ATTACH IT: Inhibition of bacterial growth) End of Lab Wrap Up Please ensure that all of your notes and materials are neatly organized to assist you in taking the end of unit assessment. Like any good scientist we expect that you will be able to use your collected data and notes to help you make amazing connections between your material. Those students with neatly organized notes will find success on the end of unit assessment. You will find that assessment located in the root folder for the unit. Please close this module and move on to the next module in this learning unit. If this is the last module, then prepare for your assessment and good luck! Protists Lab Exercise Introduction Below is a link to a Power Point that will give you an introduction this lab exercise. The introduction will provide you with important context for conducting this lab as well as clearly identify the objectives for this lab. • PowerPoint - Caption for narrations available in the notes section: Link to Protists Intro Power Point ( I will attach) Protist Diversity Exercise This Learning Module item should open in a new window. If not, then click the link below. Click to Launch ( I will attach) Plantae Lab Exercise 1 - Anatomy and Physiology Introduction Below is a link to a Power Point that will give you an introduction this lab exercise. The introduction will provide you with important context for conducting this lab as well as clearly identify the objectives for this lab. PowerPoint - Caption for narrations available in the notes section: Link to Plant Anatomy and Morphology Intro Power Point Plants play important roles in our everyday lives. Plants are shelter, medicine, clothing, and as an important food sources. Plants are also essential in the oxygen, water, and, nitrogen cycles that support life on Earth.In the distribution of life on this planet, plants provide important habitats and food sources for every animal that roams the Earth. Even if an animal doesnt depend on a plant directly for food, something in the food chain does. In our discovery of plants, we will discover various plant organs that grow both below and above the ground. Plants also have a fascinating internal anatomy. The first purpose of this lab is to familiarize you with plant cells,organs, and tissues. Secondly we hope this lab exercise will increase your appreciation for botany as food and a part of your life. ( I will attach the PPT) Materials For this exercise you will need to make an excursion to your local grocery store or to your pantry if it’s well stocked with fresh vegetables. Don’t worry you won’t be buying anything just examining them in the store (unless you want to buy them in which case you can enjoy a nice snack made of your specimens at the end of this lab). • • • You will need 8 specimens from grocery store or your pantry. You will also need to go outside and find three stems and two root systems. You will also need six different leaf types (PS - you should probably consult Google to determine what poison oak and poison ivy look like before you do this - or wear safety equipment) Remember, safety in lab is YOUR responsibility! Directions • Attached Files • Plant Anatomy and Morphology Lab.pdf Plant Anatomy and Morphology Lab.pdf - Alternative Formats (1.332 MB) Please see the attached lab document to guide you through this lab experience. ( I will attach the PDF) Materials For this lab you will need: • • Access to plants. A neighborhood walk should do fine; however, a wildlife area, a botanical garden, or even a garden store will have many of the items you are looking for. If you are unable to traverse outside for any reason you may complete this exercise using your computer and downloading images. Please either contact me or with our office of disability services to help process that accommodation. They can be reached at 972-273-3165. A digital camera (does not have to be fancy) or if you do not have one - drawing paper and lots of time. Directions Attached Files • Plant Scavenger Hunt Template.pptx Plant Scavenger Hunt Template.pptx - Alternative Formats (49.839 KB) For this lab you will be going on a plant diversity scavenger hunt/bingo. I will allow you some degree of freedom on how you upload your final product. I recommend power/point and I have provided a template for you. However, if you have another format, I will support you. The one stipulation is that it is uploaded as a file, not as a link or a shared file. If you do an amazing job your scavenger hunt may be subjected to bonus points. Step One: Go on a plant walk (or two) and see how many of these items you can find. If you are unable to find them, you can get an image from the internet but not all of your images can be from downloads. Two things here first over 50\% of your images must be original and if you do have downloads they CANNOT be from my lectures or lab materials. Step Two: for each item note (a) general location e.g. Central Park NY, NE side corner of 85th St. and 5th Ave, (b) note general observations e.g. healthy population, plentiful, raccoons were feasting on abandoned twinkles in the area, (c) indicate if the image is original or if it is downloaded - include source, and (d) general taxonomy Domain, Kingdom, Phylum and common name. Must have all four of those. Any additional info beyond that is cool! Step Three: Supplement any of your missing categories with downloads (see above, must be original and no more than 49\% of your offerings) Step Four: Put your plant scavenger hunt items into the power point template or some other creative uploadable form (PDF, PPT, PPTX or JPG only). You will upload this in the final question of your end of lab quiz. Thats it ... enjoy nature. Scavenger Hunt Checklist Y/N Location Any Bryophyte Any Fern/Fern Allies Fern Sori Any coniferophyta Any non-conifer gymnosperm A female cone Any flowering plant A monocot A eudicot Leaf with parallel venation Leaf with palmate/pinnate venation A fruit - fleshy for animal dispersal A fruit - dry Original Image Taxonomy General notes and observations 15 cm by 1 mm 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 10 11 12 13 14 15 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net 0 1 2 3 4 5 6 7 8 9 www.PrintableRulers.net Inhibition of bacterial growth Objectives • • • • Collect and analyze data to determine the effectiveness of antimicrobial agents on gram-positive and gramnegative bacterial species Apply scientific reasoning to investigate questions and utilize scientific tools to collect and analyze data (Texas ACGM Student Learning Outcomes) Use critical thinking and scientific problem solving (Texas ACGM Student Learning Outcomes) Communicate effectively the results of scientific investigations (Texas ACGM Student Learning Outcomes) Introduction Antimicrobial agents are agents that inhibit the growth of or kill bacteria. There are many different types of antimicrobial agents that are categorized into three main categories: disinfectants, antiseptics and antibiotics. Disinfectants work on inanimate objects to kill bacteria. Since they aren’t meant to be ingested or used directly on humans, they are more toxic than the other types of antimicrobial agents. Antiseptics are antimicrobial agents that are used directly on humans, so they are less toxic than disinfectants. Finally, an antibiotic works directly on bacteria, killing them by interfering with their cellular activities. They capitalize on differences between bacterial and eukaryotic cells, leaving human cells unharmed. Different antibiotics target different cellular structures, affecting bacterial species in different ways. For example, amoxicillin interferes with peptidoglycan production while erythromycin interferes with bacterial ribosomes. This activity will allow you to determine the effect that different antimicrobial agents have on the growth of two different bacterial species: Streptococcus mutans and Escherichia coli. Both species are found as a part of normal human microbiota. S. mutans is a gram-positive bacterium found in the mouth that contributes significantly to tooth decay. E. coli is a rod-shaped, gram-negative organism commonly found in the lower intestine. Though some strains have been known to cause food poisoning, most are harmless, even benefiting their host by producing vitamin K. In order to determine the effectiveness of different types of antimicrobial agents, bacteria are grown in the presence of absorbent discs that are impregnated with antimicrobial compounds. The more effective the compound is, the greater amount of inhibition to bacterial growth. This can be observed as an area of reduced bacterial growth around the disc known as a zone of inhibition (Figure 1). The size of this zone is correlated with antimicrobial activity. Figure 1 Comparison between a zone of inhibition and an untreated lawn culture Hypothesis Based on what you know about antimicrobial agents, develop a hypothesis that ranks the degree of inhibition that you expect to see from each of the three categories. Procedure Two petri dishes were inoculated with S. mutans and two with E. coli to create an even coating of cells across the surface of the agar on each plate. Seven discs impregnated with different antimicrobial agents and a control were evenly spaced across the surface of the agar on each plate. The dishes were placed in an incubator to allow the bacteria to grow and reproduce. After 24 hours, the plates were removed from the incubator and photographed for data collection. Results Print this page at actual size before taking measurements. DO NOT MEASURE FROM YOUR MONITOR. For each of the two petri dishes containing S. mutans (Figure 2), record the diameter of the zone of inhibition in millimeters on Table 1. The diameter should be measured from edge to edge across the widest point. If a zone of inhibition is not present, record the diameter as 0 mm, disregarding the diameter of the disc. For each treatment, calculate the average diameter of the zone of inhibition across the two replications. Figure 2 Two replicates of Streptococcus mutans treated with six antimicrobial agents plus a control Table 1: S. mutans 1. Antibacterial soap 2. Bleach 3. Erythromycin 4. Mouthwash 5. Amoxicillin 6. Household cleaner 7. Deionized water Category Dish 1 Dish 2 Average Using what you learned in the introduction, categorize each treatment as an antiseptic, disinfectant, antibiotic or control in ... Purchase answer to see full attachment