Python - Computer Science
Please read the FE.dox to see the 5 questions that need to be done.
Price can negotiate.
Rules: The only modules allowed for this exam are the os and re modules.
Q1) Write a program that asks the user for a file containing a FASTA nucleotide sequence (included is a file called sequence.fasta you can use). Then prompt the user to select from the following menu:
A. Calculate DNA composition: This will print to the screen the numbers of A, G, C and T n_u_c_l_e_o_t_i_d_e_s_,_ _a_n_d_ _a_n_y_ _u_n_k_n_o_w_n_s_ _(_N_’s_)_._ _
B. Calculate AT content: Prints to the screen the percentage of AT in the sequence.
C. Calculate GC content: Prints to the screen the percentage of GC in the sequence.
D. Compliment: Prints to the screen the compliment of the DNA sequence.
E. Reverse compliment: Prints to the screen the reverse compliment.
Each menu item above should be implemented in its own function. The function should be called when the user selects the respective menu item. The functions should accept as argument the DNA sequence and then perform the appropriate calculations\algorithm.
Input validation: Check to see that the file name entered by the user exists AND that the sequence is in FASTA format. You can assume that there is only one sequence in the file.
Q2) Write a program that asks the user for a file containing a FASTA nucleotide sequence (you can use the same sequence.fasta file as above). Then prompt the user to select a frame (number 1 through 6). Your program should then find the translation (protein sequence) of the nucleotide sequence in that frame. Print the translation to the screen.
Input validation: Check to see that the file name entered by the user exists AND that the sequence is in FASTA format. You can assume that there is only one sequence in the file.
Q3) Write a program that asks the user for a sequence in GenBank format (included is a file called sequence.gb that you can use). Your program should convert the GenBank formatted sequence into FASTA format. Write the FASTA formatted sequence to a file, name of which should include the accession number (i.e. NM_001250672.txt, where NM_001250672 is the accession number).
Q4) Write a program that asks the user for a file containing a nucleotide sequence AND the name of a restriction enzyme. Your program should return the positions in the sequence where the enzyme cuts. Parse out the enzymes and their cut sites from the attached RestrictionEnzymes.txt file.
Q5) Read in a whole genome (in FASTA format – _file called genome.txt, see attached) and compute the background codon frequencies. The background frequency of a codon is computed by the formula: background_frq(codon) = 100 * N(codon)/ Total_codons where N(codon) is the number of occurrence of the codon across the entire genome, and Total_codons is the total number of all codons in the whole genome. Print out the background frequency of each codon, from AAA to TTT. Use a dictionary in your solution. Your program should count codons that appear in all reading frames and then calculate and display the average.
>NM_001250672.2 Glycine max cationic peroxidase 2 (PRX2), mRNA
GAGCAAGAGTGAAGAGCGAAGAGAATGGCTCCCAAGGGTTTAATCTTTTTGGCTGTGTTATGCTTCTCAG
CACTGTCACTGAGTCGTTGTCTTGCGGAGGATAATGGACTTGTTATGAACTTCTACAAGGAATCATGCCC
TCAGGCTGAAGACATCATCAAAGAACAAGTCAAGCTTCTCTACAAGCGCCACAAGAACACTGCTTTCTCC
TGGCTCAGAAACATCTTCCATGACTGTGCTGTTCAGAGTTGTGATGCTTCACTGTTGCTGGACTCCACAA
GAAGGAGCTTGTCTGAGAAGGAAACAGATAGAAGCTTTGGGTTGAGAAATTTCAGGTACATTGAGACCAT
CAAAGAAGCTTTGGAAAGGGAATGCCCAGGAGTTGTTTCCTGTGCTGATATCCTCGTTCTCTCTGCCAGA
GATGGCATTGTTTCGCTAGGAGGTCCCCATATCCCTCTTAAAACAGGAAGAAGGGATGGTAGAAGGAGCA
GAGCCGATGTGGTAGAGCAGTTCCTCCCAGACCACAATGAATCCATTTCTGCAGTTCTTGACAAGTTTGG
TGCCATGGGAATTGACACCCCCGGCGTAGTTGCATTGCTTGGAGCACACAGTGTTGGTCGAACCCATTGT
GTGAAGTTGGTGCACCGTTTGTACCCAGAGATTGATCCAGCTCTGAACCCTGACCACGTCCCTCACATTC
TGAAGAAGTGCCCTGATGCCATTCCAGACCCTAAGGCCGTGCAGTACGTGAGAAACGACCGTGGCACCCC
CATGATTCTAGACAACAATTACTACAGAAATATATTGGACAACAAGGGCTTGTTGATAGTGGATCACCAA
CTAGCCAATGACAAGAGGACCAAGCCTTATGTGAAGAAAATGGCCAAGAGCCAGGACTATTTCTTCAAGG
AGTTTTCTAGAGCCATTACTTTGCTCTCTGAGAACAACCCTCTCACTGGCACAAAGGGTGAGATCAGAAA
GCAGTGCAATGCTGCCAACAAGCACCATGAGGAGCCTTAATTGCTTCCCGCTTAATTTGGGCCTTGAATT
TTCTTCCCCTTCTCTATGTGGAAGAAATCTGTAAGATATTATGCAAAAAATAATTAAGGTGTTTTTCTTT
AAATGGGTTGGTTGATTGGTTCAATGAACCGATCAAGACCACAGCAGGTTCATGGGGATGCGAGGATTAA
GACGCTTTGTTTTTTAATCTTCCGATGTCACTCTTGTTTGTTAGTTTGTTTTTTTATTTTTTATTTCAAT
AAGTACTGTGCAAGTAGGTTAGAGTTGGGTAGAAGGGCATGTTCATGGTGTTAATTACTATGTTATGTAT
GCATGTGAGTGCTGCTATCGATGGCAAGATGTCAATGTATGCGTGTAGTGCTGTTATCGATGAGAGTGAA
AATGTTTATGATATCCACACTAATAAAGCTAGCTTGCTCTTGCTACATAATAAATAAATCATGGCCCACG
GTCATTATACAAAAAAAAAAAAAAAAAA
AanI TTATAA
AarI CACCTGCNNNNNNNN
AasI GACNNNNNNGTC
AatI AGGCCT
AatII GACGTC
AbsI CCTCGAGG
AccI GTMKAC
AccII CGCG
AccIII TCCGGA
Acc16I TGCGCA
Acc36I ACCTGCNNNNNNNN
Acc65I GGTACC
AccB1I GGYRCC
AccB7I CCANNNNNTGG
AccBSI CCGCTC
AceIII CAGCTCNNNNNNNNNNN
AciI CCGC
AclI AACGTT
AclWI GGATCNNNNN
AcoI YGGCCR
AcsI RAATTY
AcuI CTGAAGNNNNNNNNNNNNNNNN
AcvI CACGTG
AcyI GRCGYC
AdeI CACNNNGTG
AfaI GTAC
AfeI AGCGCT
AfiI CCNNNNNNNGG
AflII CTTAAG
AflIII ACRYGT
AgeI ACCGGT
AgsI TTSAA
AhdI GACNNNNNGTC
AhlI ACTAGT
AjiI CACGTC
AjnI CCWGG
AjuI GAANNNNNNNTTGGNNNNNNNNNNN
AleI CCAANNNNNNNTTCNNNNNNNNNNNN
AlfI CACNNNNGTG
AloI GCANNNNNNTGCNNNNNNNNNNNN
AluI GAACNNNNNNTCCNNNNNNNNNNNN
AluBI GGANNNNNNGTTCNNNNNNNNNNNN
AlwI AGCT
Alw21I AGCT
Alw26I GGATCNNNNN
Alw44I GWGCWC
AlwFI GTCTCNNNNN
AlwNI GTGCAC
Ama87I GAAAYNNNNNRTG
Aor13HI CAGNNNCTG
Aor51HI CYCGRG
AoxI TCCGGA
ApaI AGCGCT
ApaBI GGCC
ApaLI GGGCCC
ApeKI GCANNNNNTGC
ApoI GTGCAC
ApyPI GCWGC
AquII RAATTY
AquIII ATCGACNNNNNNNNNNNNNNNNNNNN
AquIV GCCGNACNNNNNNNNNNNNNNNNNNNN
ArsI GAGGAGNNNNNNNNNNNNNNNNNNNN
AscI GRGGAAGNNNNNNNNNNNNNNNNNNN
AseI GACNNNNNNTTYGNNNNNNNNNNN
Asi256I CRAANNNNNNGTCNNNNNNNNNNNNN
AsiGI GGCGCGCC
AsiSI ATTAAT
AspI GATC
Asp700I ACCGGT
Asp718I GCGATCGC
AspA2I GACNNNGTC
AspCNI GAANNNNTTC
AspEI GGTACC
AspLEI CCTAGG
AspS9I GCCGC
AssI GACNNNNNGTC
AsuII GCGC
AsuC2I GGNCC
AsuHPI AGTACT
AsuNHI TTCGAA
AvaI CCSGG
AvaII GGTGANNNNNNNN
AvaIII GCTAGC
AviII CYCGRG
AvrII GGWCC
AxyI ATGCAT
BaeI TGCGCA
BaeGI CCTAGG
BalI CCTNAGG
BamHI ACNNNNGTAYCNNNNNNNNNNNN
BanI GRTACNNNNGTNNNNNNNNNNNNNNN
BanII GKGCMC
BanIII TGGCCA
BarI GGATCC
BasI GGYRCC
BauI GRGCYC
BbeI ATCGAT
Bbr7I GAAGNNNNNNTACNNNNNNNNNNNN
BbrPI GTANNNNNNCTTCNNNNNNNNNNNN
BbsI CCANNNNNTGG
BbuI CACGAG
BbvI GGCGCC
Bbv12I GAAGACNNNNNNNNNNN
BbvCI CACGTG
BccI GAAGACNNNNNN
BceAI GCATGC
BcefI GCAGCNNNNNNNNNNNN
BcgI GWGCWC
BciVI CCTCAGC
BclI CCATCNNNNN
BcnI ACGGCNNNNNNNNNNNNNN
BcuI ACGGCNNNNNNNNNNNNN
BdaI CGANNNNNNTGCNNNNNNNNNNNN
BfaI GCANNNNNNTCGNNNNNNNNNNNN
BfiI GTATCCNNNNNN
BfmI TGATCA
BfoI CCSGG
BfrI ACTAGT
BfuI TGANNNNNNTCANNNNNNNNNNNN
BfuAI CTAG
BfuCI ACTGGGNNNNN
BglI CTRYAG
BglII RGCGCY
BisI CTTAAG
BlnI GTATCCNNNNNN
BlpI ACCTGCNNNNNNNN
BlsI GATC
BmcAI GCCNNNNNGGC
Bme18I AGATCT
Bme1390I GCNGC
BmeRI CCTAGG
BmeT110I GCTNAGC
BmgI GCNGC
BmgBI AGTACT
BmgT120I GGWCC
BmiI CCNGG
BmrI GACNNNNNGTC
BmrFI CYCGRG
BmsI GKGCCC
BmtI CACGTC
BmuI GGNCC
BoxI GGNNCC
BpiI ACTGGGNNNNN
BplI CCNGG
BpmI GCATCNNNNNNNNN
Bpu10I GCTAGC
Bpu14I ACTGGGNNNNN
Bpu1102I GACNNNNGTC
BpuAI GAAGACNNNNNN
BpuEI GAGNNNNNCTCNNNNNNNNNNNNN
BpuMI CTGGAGNNNNNNNNNNNNNNNN
BpvUI CCTNAGC
BsaI TTCGAA
Bsa29I GCTNAGC
BsaAI GAAGACNNNNNN
BsaBI CTTGAGNNNNNNNNNNNNNNNN
BsaHI CCSGG
BsaJI CGATCG
BsaMI GGTCTCNNNNN
BsaWI ATCGAT
BsaXI YACGTR
BsbI GATNNNNATC
Bsc4I GRCGYC
BscAI CCNNGG
BscGI GAATGCN
Bse1I WCCGGW
Bse8I ACNNNNNCTCCNNNNNNNNNN
Bse21I GGAGNNNNNGTNNNNNNNNNNNN
Bse118I CAACACNNNNNNNNNNNNNNNNNNNNN
BseAI CCNNNNNNNGG
BseBI GCATCNNNNNN
BseCI CCCGT
BseDI ACTGGN
Bse3DI GATNNNNATC
BseGI CCTNAGG
BseJI RCCGGY
BseLI TCCGGA
BseMI CCWGG
BseMII ATCGAT
BseNI CCNNGG
BsePI GCAATGNN
BseRI GGATGNN
BseSI GATNNNNATC
BseXI CCNNNNNNNGG
BseX3I GCAATGNN
BseYI CTCAGNNNNNNNNNN
BsgI ACTGGN
Bsh1236I GCGCGC
Bsh1285I GAGGAGNNNNNNNNNN
BshFI GKGCMC
BshNI GCAGCNNNNNNNNNNNN
BshTI CGGCCG
BshVI CCCAGC
BsiEI GTGCAGNNNNNNNNNNNNNNNN
BsiHKAI CGCG
BsiHKCI CGRYCG
BsiSI GGCC
BsiWI GGYRCC
BslI ACCGGT
BslFI ATCGAT
BsmI CGRYCG
BsmAI GWGCWC
BsmBI CYCGRG
BsmFI CCGG
BsnI CGTACG
Bso31I CCNNNNNNNGG
BsoBI GGGACNNNNNNNNNNNNNN
Bsp13I GAATGCN
Bsp19I GTCTCNNNNN
Bsp24I CGTCTCNNNNN
Bsp68I GGGACNNNNNNNNNNNNNN
Bsp119I GGCC
Bsp120I GGTCTCNNNNN
Bsp143I CYCGRG
Bsp1286I TCCGGA
Bsp1407I CCATGG
Bsp1720I GACNNNNNNTGGNNNNNNNNNNNN
BspACI CCANNNNNNGTCNNNNNNNNNNNNN
BspCNI TCGCGA
BspDI TTCGAA
BspD6I GGGCCC
BspEI GATC
BspFNI GDGCHC
BspGI TGTACA
BspHI GCTNAGC
BspLI CCGC
BspMI CTCAGNNNNNNNNN
BspNCI ATCGAT
BspOI GACTCNNNNNN
BspPI TCCGGA
BspQI CGCG
BspTI CTGGAC
BspT104I TCATGA
BspT107I GGNNCC
BspTNI ACCTGCNNNNNNNN
BsrI CCAGA
BsrBI GCTAGC
BsrDI GGATCNNNNN
BsrFI GCTCTTCNNNN
BsrGI CTTAAG
BsrSI TTCGAA
BssAI GGYRCC
BssECI GGTCTCNNNNN
BssHII ACTGGN
BssKI CCGCTC
BssMI GCAATGNN
BssNI RCCGGY
BssNAI TGTACA
BssSI ACTGGN
BssT1I RCCGGY
Bst6I CCNNGG
Bst98I GCGCGC
Bst1107I CCNGG
BstACI GATC
BstAFI GRCGYC
BstAPI GTATAC
BstAUI CACGAG
BstBI CCWWGG
Bst2BI CTCTTCNNNN
BstBAI CTTAAG
Bst4CI GTATAC
BstC8I GRCGYC
BstDEI CTTAAG
BstDSI GCANNNNNTGC
BstEII TGTACA
BstENI TTCGAA
BstF5I CACGAG
BstFNI YACGTR
BstH2I ACNGT
BstHHI GCNNGC
BstKTI CTNAG
BstMAI CCRYGG
BstMBI GGTNACC
BstMCI CCTNNNNNAGG
BstMWI GGATGNN
BstNI CGCG
BstNSI RGCGCY
BstOI GCGC
BstPI GATC
BstPAI GTCTCNNNNN
BstSCI GATC
BstSFI CGRYCG
BstSLI GCNNNNNNNGC
BstSNI CCWGG
BstUI RCATGY
Bst2UI CCWGG
BstV1I GGTNACC
BstV2I GACNNNNGTC
BstXI CCNGG
BstX2I CTRYAG
BstYI GKGCMC
BstZI TACGTA
BstZ17I CGCG
BsuI CCWGG
Bsu15I GCAGCNNNNNNNNNNNN
Bsu36I GAAGACNNNNNN
BsuRI CCANNNNNNTGG
BsuTUI RGATCY
BtgI RGATCY
BtgZI CGGCCG
BthCI GTATAC
BtrI GTATCCNNNNNN
BtsI ATCGAT
BtsCI CCTNAGG
BtuMI GGCC
BveI ATCGAT
Cac8I CCRYGG
CaiI GCGATGNNNNNNNNNNNNNN
CciI GCNGC
CciNI CACGTC
CdiI GCAGTGNN
CdpI GGATGNN
CelII TCGCGA
CfoI ACCTGCNNNNNNNN
CfrI GCNNGC
Cfr9I CAGNNNCTG
Cfr10I TCATGA
Cfr13I GCGGCCGC
Cfr42I CATCG
ChaI GCGGAGNNNNNNNNNNNNNNNNNNNN
CjeI GCTNAGC
CjeNII GCGC
CjePI YGGCCR
CjeP659IV CCCGGG
CjuI RCCGGY
CjuII GGNCC
ClaI CCGCGG
CpoI GATC
CseI CCANNNNNNGTNNNNNNNNNNNNNNN
CsiI ACNNNNNNTGGNNNNNNNNNNNNNN
CspI GAGNNNNNGT
Csp6I CCANNNNNNNTCNNNNNNNNNNNNNN
Csp45I GANNNNNNNTGGNNNNNNNNNNNNN
CspAI CACNNNNNNNGAA
CspCI CAYNNNNNRTG
CstMI CAYNNNNNCTC
CviAII ATCGAT
CviJI CGGWCCG
CviKI-1 GACGCNNNNNNNNNN
CviQI ACCWGGT
DdeI CGGWCCG
DinI GTAC
DpnI TTCGAA
DpnII ACCGGT
DraI CAANNNNNGTGGNNNNNNNNNNNN
DraII CCACNNNNNTTGNNNNNNNNNNNNN
DraIII AAGGAGNNNNNNNNNNNNNNNNNNNN
DraRI CATG
DrdI RGCY
DrdII RGCY
DrdIV GTAC
DriI CTNAG
DseDI GGCGCC
EaeI GATC
EagI GATC
Eam1104I TTTAAA
Eam1105I RGGNCCY
EarI CACNNNGTG
EciI CAAGNACNNNNNNNNNNNNNNNNNNNN
Ecl136II GACNNNNNNGTC
EclXI GAACCA
Eco24I TACGACNNNNNNNNNNNNNNNNNNNN
Eco31I GACNNNNNGTC
Eco32I GACNNNNNNGTC
Eco47I YGGCCR
Eco47III CGGCCG
Eco52I CTCTTCNNNN
Eco57I GACNNNNNGTC
Eco72I CTCTTCNNNN
Eco81I GGCGGANNNNNNNNNNN
Eco88I GAGCTC
Eco91I CGGCCG
Eco105I GRGCYC
Eco130I GGTCTCNNNNN
Eco147I GATATC
EcoHI GGWCC
EcoICRI AGCGCT
Eco57MI CGGCCG
EcoNI CTGAAGNNNNNNNNNNNNNNNN
EcoO65I CACGTG
EcoO109I CCTNAGG
EcoRI CYCGRG
EcoRII GGTNACC
EcoRV TACGTA
EcoT14I CCWWGG
EcoT22I AGGCCT
EcoT38I CCSGG
Eco53kI GAGCTC
EgeI CTGRAGNNNNNNNNNNNNNNNN
EheI CCTNNNNNAGG
ErhI GGTNACC
EsaBC3I RGGNCCY
EsaSSI GAATTC
Esp3I CCWGG
FaeI GATATC
FaiI CCWWGG
FalI ATGCAT
FaqI GRGCYC
FatI GAGCTC
FauI GGCGCC
FauNDI GGCGCC
FbaI CCWWGG
FblI TCGA
FinI GACCAC
FmuI CGTCTCNNNNN
Fnu4HI CATG
FokI YATR
FriOI AAGNNNNNCTTNNNNNNNNNNNNN
FseI GGGACNNNNNNNNNNNNNN
FspI CATG
FspAI CCCGCNNNNNN
FspBI CATATG
FspEI TGATCA
Fsp4HI GTMKAC
GdiII GGGAC
GlaI GGNCC
GluI GCNGC
GsaI GGATGNNNNNNNNNNNNN
GsuI GRGCYC
HaeI GGCCGGCC
HaeII TGCGCA
HaeIII RTGCGCAY
HaeIV CTAG
HapII CCNNNNNNNNNNNNNNNN
HgaI GCNGC
HgiEII CGGCCR
HhaI GCGC
Hin1I GCNGC
Hin1II CCCAGC
Hin4I CTGGAGNNNNNNNNNNNNNNNN
Hin6I WGGCCW
HinP1I RGCGCY
HincII GGCC
HindII GAYNNNNNRTCNNNNNNNNNNNNNN
HindIII GAYNNNNNRTCNNNNNNNNNNNNN
HinfI CCGG
HpaI GACGCNNNNNNNNNN
HpaII ACCNNNNNNGGT
HphI GCGC
Hpy8I GRCGYC
Hpy99I CATG
Hpy166II GAYNNNNNVTCNNNNNNNNNNNNN
Hpy188I GABNNNNNRTCNNNNNNNNNNNNN
Hpy188III GCGC
HpyAV GCGC
HpyCH4III GTYRAC
HpyCH4IV GTYRAC
HpyCH4V AAGCTT
HpyF3I GANTC
HpyF10VI GTTAAC
Hsp92I CCGG
Hsp92II GGTGANNNNNNNN
HspAI GTNNAC
KasI CGWCG
KflI GTNNAC
KpnI TCNGA
Kpn2I TCNNGA
KspI CCTTCNNNNNN
Ksp22I ACNGT
KspAI ACGT
Kzo9I TGCA
LguI CTNAG
LlaGI GCNNNNNNNGC
LpnI GRCGYC
LpnPI CATG
Lsp1109I GCGC
LweI GGCGCC
MabI GGGWCCC
MaeI GGTACC
MaeII TCCGGA
MaeIII CCGCGG
MalI TGATCA
MaqI GTTAAC
MauBI GATC
MbiI GCTCTTCNNNN
MboI CTNGAYG
MboII RGCGCY
McaTI CCDGNNNNNNNNNNNNNN
MfeI GCAGCNNNNNNNNNNNN
MflI GCATCNNNNNNNNN
MhlI ACCWGGT
MjaIV CTAG
MlsI ACGT
MluI GTNAC
MluNI GATC
MlyI CRTTGACNNNNNNNNNNNNNNNNNNNNN
Mly113I CGCGCGCG
MmeI CCGCTC
MnlI GATC
Mph1103I GAAGANNNNNNNN
MreI GCGCGC
MroI CAATTG
MroNI RGATCY
MroXI GDGCHC
MscI GTNNAC
MseI TGGCCA
MslI ACGCGT
MspI TGGCCA
Msp20I GAGTCNNNNN
MspA1I GGCGCC
MspCI TCCRACNNNNNNNNNNNNNNNNNNNN
MspJI CCTCNNNNNNN
MspR9I ATGCAT
MssI CGCCGGCG
MunI TCCGGA
MvaI GCCGGC
Mva1269I GAANNNNTTC
MvnI TGGCCA
MvrI TTAA
MwoI CAYNNNNRTG
NaeI CCGG
NarI TGGCCA
NciI CMGCKG
NcoI CTTAAG
NdeI CNNRNNNNNNNNNNNNN
NdeII CCNGG
NgoAVIII GTTTAAAC
NgoMIV CAATTG
NhaXI CCWGG
NheI GAATGCN
NlaIII CGCG
NlaIV CGATCG
NlaCI GCNNNNNNNGC
Nli3877I GCCGGC
NmeAIII GGCGCC
NmeDI CCSGG
NmuCI CCATGG
NotI CATATG
NruI GATC
NsbI GACNNNNNTGANNNNNNNNNNNNN
NsiI TCANNNNNGTCNNNNNNNNNNNNNN
NspI GCCGGC
NspV CAAGRAG
OliI GCTAGC
PabI CATG
PacI GGNNCC
PaeI CATCACNNNNNNNNNNNNNNNNNNN
PaeR7I CYCGRG
PagI GCCGAGNNNNNNNNNNNNNNNNNNNNN
PalAI RCCGGYNNNNNNNNNNNN
PasI GTSAC
PauI GCGGCCGC
PceI TCGCGA
PciI TGCGCA
PciSI ATGCAT
PcsI RCATGY
PctI TTCGAA
PdiI CACNNNNGTG
PdmI GTAC
PfeI TTAATTAA
Pfl23II GCATGC
Pfl1108I CTCGAG
PflFI TCATGA
PflMI GGCGCGCC
PfoI CCCWGGG
PhoI GCGCGC
PinAI AGGCCT
PlaDI ACATGT
PleI GCTCTTCNNNN
Ple19I WCGNNNNNNNCGW
PmaCI GAATGCN
PmeI GCCGGC
PmlI GAANNNNTTC
PpiI GAWTC
PpsI CGTACG
Ppu10I TCGTAG
Ppu21I GACNNNGTC
PpuMI CCANNNNNTGG
PscI TCCNGGA
PshAI GGCC
PshBI ACCGGT
PsiI CATCAGNNNNNNNNNNNNNNNNNNNNN
Psp03I GAGTCNNNNN
Psp5II CGATCG
Psp6I CACGTG
Psp1406I GTTTAAAC
Psp124BI CACGTG
PspCI GAACNNNNNCTCNNNNNNNNNNNNN
PspEI GAGNNNNNGTTCNNNNNNNNNNNN
PspGI GAGTCNNNNN
PspLI ATGCAT
PspN4I YACGTR
PspOMI RGGWCCY
PspOMII ACATGT
PspPI GACNNNNGTC
PspPPI ATTAAT
PspPRI TTATAA
PspXI GGWCC
PsrI RGGWCCY
PssI CCWGG
PstI AACGTT
PstNI GAGCTC
PsuI CACGTG
PsyI GGTNACC
PteI CCWGG
PvuI CGTACG
PvuII GGNNCC
RcaI GGGCCC
RceI CGCCCARNNNNNNNNNNNNNNNNNNNN
RgaI GGNCC
RigI RGGWCCY
RleAI CCYCAGNNNNNNNNNNNNNNN
RpaBI VCTCGAGB
RpaB5I GAACNNNNNNTACNNNNNNNNNNNN
RruI GTANNNNNNGTTCNNNNNNNNNNNN
RsaI RGGNCCY
RsaNI CTGCAG
RseI CAGNNNCTG
RsrII RGATCY
Rsr2I GACNNNGTC
SacI GCGCGC
SacII CGATCG
SalI CAGCTG
SapI TCATGA
SaqAI CATCGACNNNNNNNNNNNNNNNNNNNN
SatI GCGATCGC
Sau96I GGCCGGCC
Sau3AI CCCACANNNNNNNNNNNN
SbfI CCCGCAGNNNNNNNNNNNNNNNNNNNN
ScaI CGRGGACNNNNNNNNNNNNNNNNNNNN
SchI TCGCGA
SciI GTAC
ScrFI GTAC
SdaI CAYNNNNRTG
SdeAI CGGWCCG
SdeOSI CGGWCCG
SduI GAGCTC
SelI CCGCGG
SetI GTCGAC
SexAI GCTCTTCNNNN
SfaAI TTAA
SfaNI GCNGC
SfcI GGNCC
SfiI GATC
SfoI CCTGCAGG
Sfr274I AGTACT
Sfr303I GAGTCNNNNN
SfuI CTCGAG
SgeI CCNGG
SgfI CCTGCAGG
SgrAI CAGRAGNNNNNNNNNNNNNNNNNNNNN
SgrBI GACNNNNRTGANNNNNNNNNNNN
SgrDI TCAYNNNNGTCNNNNNNNNNNNNN
SgsI GDGCHC
SimI CGCG
SinI ASST
SlaI ACCWGGT
SmaI GCGATCGC
SmiI GCATCNNNNNNNNN
SmiMI CTRYAG
SmlI GGCCNNNNNGGCC
SmoI GGCGCC
SmuI CTCGAG
SnaI CCGCGG
SnaBI TTCGAA
SpeI CNNGNNNNNNNNNNNNN
SphI GCGATCGC
SpoDI CRCCGGYG
SrfI CCGCGG
Sse9I CGTCGACG
Sse8387I GGCGCGCC
Sse8647I GGGTC
SseBI GGWCC
SsiI CTCGAG
SspI CCCGGG
SspDI ATTTAAAT
SspD5I CAYNNNNRTG
SstI CTYRAG
SstII CTYRAG
SstE37I CCCGCNNNNNN
Sth132I GTATAC
Sth302II TACGTA
StrI ACTAGT
StsI GCATGC
StuI GCGGRAG
StyI GCCCGGGC
StyD4I AATT
SwaI CCTGCAGG
TaaI AGGWCCT
TaiI AGGCCT
TaqI CCGC
TaqII AATATT
TasI GGCGCC
TatI GGTGANNNNNNNN
TauI GAGCTC
TfiI CCGCGG
TliI CGAAGACNNNNNNNNNNNNNNNNNNNN
Tru1I CCCGNNNNNNNN
Tru9I CCGG
TscAI CTCGAG
TseI GGATGNNNNNNNNNNNNNN
TsoI AGGCCT
Tsp45I CCWWGG
Tsp509I CCNGG
TspDTI ATTTAAAT
TspEI ACNGT
TspGWI ACGT
TspMI TCGA
TspRI GACCGANNNNNNNNNNN
TssI CACCCANNNNNNNNNNN
TstI AATT
TsuI WGTACW
Tth111I GCSGC
Tth111II GAWTC
UbaF9I CTCGAG
UbaF11I TTAA
UbaF12I TTAA
UbaF13I CASTGNN
UbaF14I GCWGC
UbaPI TARCCANNNNNNNNNNN
UnbI GTSAC
Van91I AATT
Vha464I ATGAANNNNNNNNNNN
VneI AATT
VpaK11AI ACGGANNNNNNNNNNN
VpaK11BI CCCGGG
VspI CASTGNN
XagI GAGNNNCTC
XapI CACNNNNNNTCCNNNNNNNNNNNN
XbaI GGANNNNNNGTGNNNNNNNNNNNNN
XceI GCGAC
XcmI GACNNNGTC
XhoI CAARCANNNNNNNNNNN
XhoII TACNNNNNRTGT
XmaI TCGTA
XmaJI CTACNNNGTC
XmiI GAGNNNNNNCTGG
XmnI CCANNNNNTCG
XspI CGAACG
ZraI GGNCC
ZrmI CCANNNNNTGG
Zsp2I CTTAAG
…
LOCUS NM_001250672 1498 bp mRNA linear PLN 18-OCT-2018
DEFINITION Glycine max cationic peroxidase 2 (PRX2), mRNA.
ACCESSION NM_001250672
VERSION NM_001250672.2
KEYWORDS RefSeq.
SOURCE Glycine max (soybean)
ORGANISM Glycine max
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50
kb inversion clade; NPAAA clade; indigoferoid/millettioid clade;
Phaseoleae; Glycine; Glycine subgen. Soja.
REFERENCE 1 (bases 1 to 1498)
AUTHORS Gijzen M, Miller SS, Bowman LA, Batchelor AK, Boutilier K and Miki
BL.
TITLE Localization of peroxidase mRNAs in soybean seeds by in situ
hybridization
JOURNAL Plant Mol. Biol. 41 (1), 57-63 (1999)
PUBMED 10561068
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from BT099403.1.
On Sep 4, 2012 this sequence version replaced NM_001250672.1.
##Evidence-Data-START##
Transcript exon combination :: AK244214.1, AK286032.1 [ECO:0000332]
RNAseq introns :: single sample supports all introns
SAMN00264986, SAMN00264988
[ECO:0000348]
##Evidence-Data-END##
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-1498 BT099403.1 1-1498 c
FEATURES Location/Qualifiers
source 1..1498
/organism=Glycine max
/mol_type=mRNA
/db_xref=taxon:3847
/chromosome=17
/map=17
gene 1..1498
/gene=PRX2
/note=cationic peroxidase 2
/db_xref=GeneID:547513
misc_feature 10..12
/gene=PRX2
/note=upstream in-frame stop codon
CDS 25..1020
/gene=PRX2
/EC_number=1.11.1.7
/note=class III plant peroxidase
/codon_start=1
/product=cationic peroxidase 2 precursor
/protein_id=NP_001237601.1
/db_xref=GeneID:547513
/translation=MAPKGLIFLAVLCFSALSLSRCLAEDNGLVMNFYKESCPQAEDI
IKEQVKLLYKRHKNTAFSWLRNIFHDCAVQSCDASLLLDSTRRSLSEKETDRSFGLRN
FRYIETIKEALERECPGVVSCADILVLSARDGIVSLGGPHIPLKTGRRDGRRSRADVV
EQFLPDHNESISAVLDKFGAMGIDTPGVVALLGAHSVGRTHCVKLVHRLYPEIDPALN
PDHVPHILKKCPDAIPDPKAVQYVRNDRGTPMILDNNYYRNILDNKGLLIVDHQLAND
KRTKPYVKKMAKSQDYFFKEFSRAITLLSENNPLTGTKGEIRKQCNAANKHHEEP
sig_peptide 25..96
/gene=PRX2
/inference=COORDINATES: ab initio prediction:SignalP:4.0
ORIGIN
1 gagcaagagt gaagagcgaa gagaatggct cccaagggtt taatcttttt ggctgtgtta
61 tgcttctcag cactgtcact gagtcgttgt cttgcggagg ataatggact tgttatgaac
121 ttctacaagg aatcatgccc tcaggctgaa gacatcatca aagaacaagt caagcttctc
181 tacaagcgcc acaagaacac tgctttctcc tggctcagaa acatcttcca tgactgtgct
241 gttcagagtt gtgatgcttc actgttgctg gactccacaa gaaggagctt gtctgagaag
301 gaaacagata gaagctttgg gttgagaaat ttcaggtaca ttgagaccat caaagaagct
361 ttggaaaggg aatgcccagg agttgtttcc tgtgctgata tcctcgttct ctctgccaga
421 gatggcattg tttcgctagg aggtccccat atccctctta aaacaggaag aagggatggt
481 agaaggagca gagccgatgt ggtagagcag ttcctcccag accacaatga atccatttct
541 gcagttcttg acaagtttgg tgccatggga attgacaccc ccggcgtagt tgcattgctt
601 ggagcacaca gtgttggtcg aacccattgt gtgaagttgg tgcaccgttt gtacccagag
661 attgatccag ctctgaaccc tgaccacgtc cctcacattc tgaagaagtg ccctgatgcc
721 attccagacc ctaaggccgt gcagtacgtg agaaacgacc gtggcacccc catgattcta
781 gacaacaatt actacagaaa tatattggac aacaagggct tgttgatagt ggatcaccaa
841 ctagccaatg acaagaggac caagccttat gtgaagaaaa tggccaagag ccaggactat
901 ttcttcaagg agttttctag agccattact ttgctctctg agaacaaccc tctcactggc
961 acaaagggtg agatcagaaa gcagtgcaat gctgccaaca agcaccatga ggagccttaa
1021 ttgcttcccg cttaatttgg gccttgaatt ttcttcccct tctctatgtg gaagaaatct
1081 gtaagatatt atgcaaaaaa taattaaggt gtttttcttt aaatgggttg gttgattggt
1141 tcaatgaacc gatcaagacc acagcaggtt catggggatg cgaggattaa gacgctttgt
1201 tttttaatct tccgatgtca ctcttgtttg ttagtttgtt tttttatttt ttatttcaat
1261 aagtactgtg caagtaggtt agagttgggt agaagggcat gttcatggtg ttaattacta
1321 tgttatgtat gcatgtgagt gctgctatcg atggcaagat gtcaatgtat gcgtgtagtg
1381 ctgttatcga tgagagtgaa aatgtttatg atatccacac taataaagct agcttgctct
1441 tgctacataa taaataaatc atggcccacg gtcattatac aaaaaaaaaa aaaaaaaa
//
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